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Microsomal Stability

Fast, Accurate and Affordable…

We understand that your timelines and budgets are tight so we’ve coupled high resolution accurate mass Q-TOF MS with HPLC to help you do more with less. Full-scan data is acquired, from which narrow window extracted ion chromatograms are generated, producing quantitative data equivalent to that obtained by HPLC/MS/MS without the time consuming process of developing distinct, MS methods for each test article. The streamlined work flow means data is returned to you within 72 hours enabling you to guide structural modifications, predict in vivo performance, develop structure-metabolic stability relationships and triage compounds for further studies in real time.

In vitro data is often ambiguous due to low compound solubility frequently observed during the discovery process. The use of a cosolvent minimizes precipitation and reduces non-specific binding to plastics allowing accurate data to be delivered for a higher percentage of compounds.

Bonus: Metabolic route information can be derived from further mining the acquired LC-TOFMS datasets.

Assay Protocol

Compound Requirements

  • 10 µL of 10mM stock solution

Test Compound Concentration

  • 1-5µM

Microsome Concentration

  • 0.5mg/mL (other concentrations available)

Time Points

  • Screening: 0 and 15, or 30, or 60 min
  • in vitro t½: 0, 5, 10, 30, 60



Final Cosolvent Concentration

  • 0.2% DMSO
  • 0.8% Acetonitrile

Analysis Method

  • LC-TOFMS – full scan accurate mass data is acquired

Data Delivery

  • Delivered within 72 hours
  • Screening: % Remaining
  • in vitro t½: Half Life, Intrinsic Clearance


  • Screening: $150.00
  • In Vitro t½: $350.00
72 Hours
Guaranteed turn around time after receiving your compound
starting price
of 10mM DMSO stock solution

Physchem Services •  LogD / Log P •  Aqueous Solubility •  pKa •  Chemical Stability •  XRPD •  Accurate Mass
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