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Solubility

Fast, Accurate and Affordable…

Whether you’re tight on budget, time, or internal capacity Analiza offers a solubility assay to meet your needs. We use only miniaturized, gold- standard shake-flask assays with quantitation by LC-MS, LC-UV or chemiluminscent nitrogen detection (CLND) to measure both kinetic (from DMSO stock solutions) and thermodynamic (from dry powder) solubility.

For early screening we’ve applied the equimolar detection capabilities of CLND. Eliminating the need for compound specific calibration, we’re able to provide accurate, shake-flask solubility measurements with just 10 µL of DMSO stock solution. This method is best suited for rank ordering compounds, predicting solubility for in vitro biology experiments and whenever sample is at a premium.

When your solubility assay demands high sensitivity (sub-micromolar-nanomolar) or absolute integrity we employ our state of the art, Ultra-High Accuracy QTOF-MS for analysis. With sub 2ppm mass accuracy, the QTOF-MS provides absolute identification of the test article and when operated in full scan auto ms-ms mode also allows for the identification of impurities and degradation products.

As compounds progress through the discovery cycle and more rigorous thermodynamic solubility measurements are needed we offer the same miniaturized shake-flask method with quantitation by LC-MS, LC-UV.

Let us leverage our experience with hundreds of thousands of compounds to design a screening strategy that is right for you. If you already have a program in place but don’t have available internal resources to keep up we can assay your samples under a method transfer agreement.


Assay Protocol

Method

  • Miniaturized Shake Flask

Compound Requirements

  • Kinetic: 10 µL (CLND), 20 µL (LC-UV or LC-MS)
  • Thermodynamic: 0.5 mg of dry powder

Dose Concentration

  • Kinetic: 200 µM (others available)
  • Thermodynamic: 2 mg/mL (others available)

Incubation Time

  • 24 hours (others available)

Assay Media

  • SGF, pH 1.2
  • SIF, pH 6.5
  • PBS, pH 7.4
  • Other media available

LOQ

  • 1 µM, CLND and HPLC-UV
  • 0.001µM, LC-MS

Quantitation Method

  • CLND
  • HPLC-UV

Data Delivery within 72 hours

  • Solubility (µM and µg/mL)

Cost

  • CLND: $25.00 per compound per media
  • HPLC-UV: $125.00 per compound
    • $25.00 each additional media or repeat
  • LC-MS: $150.00 per compound
    • $50.00 each additional media or repeat
At-a-glance
Target
Method
Miniaturized Shake Flask: Kinetic and Thermodynamic Solubility
Target
72 Hours
Guaranteed turn around time after receiving your compound
Target
$25
starting price
Target
10µL
of 10mM DMSO stock solution
Target
<5%
Intra-Assay CV

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