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Log D/Log P

Fast, Accurate and Affordable…

Whether you’re tight on budget, time, or internal capacity Analiza offers a Log D/Log P assay to meet your needs. We understand the fast timelines and scarce compound availability associated with discovery operations so we scaled down the gold-standard shake-flask method to deliver accurate, uncompromised data from just 30 µL of 10mM DMSO stock solutions in 72 hours or less.

Single point assays provide robust rank order classification of compounds under physiologically relevant conditions. For a more thorough investigation into their in vitro behavior our vast library of octanol-buffer partitioning systems composed of a universal buffer in equilibrium with octanol allows us to quickly and affordably deliver true shake-flask pH-Log D profiles over the entire physiologically relevant pH range without buffer composition artifacts.

Using measured pKa data, Log P is determined under conditions at which the compound is neutral.

Let us leverage our experience with tens of thousands of compounds to design a screening strategy that is right for you.


Assay Protocol

Method

  • Miniaturized Shake-Flask, octanol in equilibrium with buffer

Compound Requirements

  • 30 µL of 10mM DMSO stock solution

Dose Concentration

  • Single Point: 1 mM (others available)
  • 3 Pt Conc. Dependence: 0.1, 0.5, 0.01 mM

Assay Buffer Composition

  • Single Point: PBS, pH 7.4 (others available)
  • 4 Pt. pH-LogD Profile: Universal Buffer, pH 2, 4, 7.4, 9 (others available)

Analytical Range

  • -3 = LogD/P = 4

Quantitation Method

  • CLND
  • Data Delivery within 72 hours
  • Log D/Log P

Cost

  • Single Pt: $25.00 per compound per media
  • 4 Pt. pH-LogD Profile: $100.00 per compound
  • 3 Pt. Conc. Dependence: $75.00 per compound per media
At-a-glance
Target
Method
Miniaturized Shake Flask: Octanol in equilibrium with buffer
Target
72 Hours
Guaranteed turn around time after receiving your compound
Target
$25
starting price
Target
30µL
of 10mM DMSO stock solution
Target
<5%
Intra-Assay CV

Physchem Services •  LogD / Log P •  Aqueous Solubility •  pKa •  Chemical Stability •  XRPD •  Accurate Mass
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