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pKa

Fast, Accurate and Affordable…

We scaled back the compound requirements to deliver accurate pKa data from just 25 µL of 10mM DMSO stock solution because we understand that you can’t spare mg’s of material for a single measurement during the discovery phase. We also understand that the timescale for discovery options is measured in hours not days or weeks so we guarantee that data is delivered within 72 hours.

Capillary electrophoresis separations are carried out in parallel across 24 equally spaced pH’s between 1.8 and 11.2. The compound migration time is monitored relative to a neutral marker (DMSO) and a titration curve is generated from this data.


Assay Protocol

Compound Requirements

  • 25 µL of 10mM DMSO stock solution

Test Compound Concentration

  • 100 µM

Analytical Range

  • pKa 1.8-11.2

Resolution

  • 0.5 pH units

Analysis Method

  • pKa PRO Analyzer (AATI): 24 point parallel capillary electrophoresis with quantitation by UV absorbance at 229nm.
  • Aqueous: Intrinsic Solubility >10 µg/mL
  • Co-solvent Intrinsic Solubility >1 µg/mL

Data Delivery within 72 hours

  • pKa(s)
  • 24 point titration curve

Cost

  • $125.00 per compound
At-a-glance
pKa
Target
Method
24 Point Parallel Capillary Electrophoresis
Target
72 Hours
Guaranteed turn around time after receiving your compound
Target
$125
starting price
Target
25µL
of 10mM DMSO stock solution
Target
<2%
Intra-Assay CV

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