PAMPAADME Services

pampa

Focused on early discovery, we understand that compounds at this stage often suffer from low aqueous solubility and high non-specific binding. That’s why we don’t assume that compounds are fully soluble under the assay conditions, nor do we rely on assumed mass balance.  Instead, with as little as 20 μL of 10 mM DMSO stock solution, we measure actual dose concentration at T0 along with the concentration in the donor and acceptor wells. The PAMPA assay generates consistent, reproducible data over a range of permeability values.

To avoid non-specific binding, we’ve incorporated Corning GentestTM pre-coated PAMPA plates into our workflow.  Unlike traditional PAMPA formulations, these plates are comprised of an oil-lipid-oil trilayer, offering a shortened path for compound diffusion more closely mimicking diffusion across the cell membrane while minimizing non-specific binding and improving the accuracy of PAMPA permeability values for compounds that are typically under-predicted using traditional PAMPA methods.

Ask us about obtaining solubility data alongside PAMPA for a more clear picture of your sample’s properties.

assay requirements

Method

  • Parallel Artificial Membrane Permeability using the Corning GentestTM pre-coated PAMPA plate system.

Compound Requirements

  • 20 μL of 10 mM DMSO stock (HPLC-UV, HPLC-MS, and CLND)

Dose Concentration

  • 200 μM (HPLC-UV and CLND)
  • 10 μM (HPLC-MS)

Incubation Time

  • 5 hours (others available)

Assay Media

  • PBS, pH 7.4
  • Other media available

Quantitation Method

  • CLND
  • HPLC-UV
  • HPLC-MS

Data Delivery

  • Papp
  • C0
  • Percent Retained

Cost

  • Quotes available upon request

our experts are available to answer your questions

Demos

Color Skin

Header Style

Nav Mode

Layout

Wide
Boxed

Nav Mode