Microsomal StabilityADME Services

Microsomal Stability

Timelines and budgets are tight, so we’ve coupled high resolution accurate mass Q-TOF microsomal stability with HPLC to help you do more with less. Full-scan data is acquired, from which narrow window extracted ion chromatograms are generated, producing quantitative data equivalent to that obtained by HPLC/MS/MS without the time-consuming process of developing distinct,  MS methods for each test article. 

Our streamlined work flow means data is returned to you in as little as 72 hours, enabling you to guide structural modifications, predict in vivo performance, develop structure-metabolic stability relationships, and triage compounds for further studies in real time.

In vitro data is often ambiguous due to low compound solubility frequently observed during the discovery process.  The use of a cosolvent in our microsomal stability assay minimizes precipitation and reduces non-specific binding to plastics, allowing accurate data to be delivered for a higher percentage of compounds.

assay requirements

Compound Requirements

  • 10 μL of 10 mM stock solution

Test Compound Concentration

  • 1-5 μM

Microsome Concentration

  • 0.5 mg/mL (other concentrations available)

Time Points

  • Screening: 0 and 15, or 30, or 60 min.
  • In vitro t1/2: 0, 5, 15, 30, and 60 min.



Final Cosolvent Concentration

  • 0.2% DMSO
  • 0.8% Acetonitrile

Analysis Method

  • HPLC-MS – full scan accurate mass data is acquired

Data Delivery

  • Screening: Percent Remaining
  • In vitro t1/2: Half Life, Intrinsic Clearance


Quotes available upon request for:

  • Screening
  • In vitro t1/2

our experts are available to answer your questions