Focused on early discovery, we understand that compounds at this stage often suffer from low aqueous solubility and high non-specific binding. That’s why we don’t assume that compounds are fully soluble under the assay conditions, nor do we rely on assumed mass balance. Instead, with as little as 20 μL of 10 mM DMSO stock solution, we measure actual dose concentration at T0 along with the concentration in the donor and acceptor wells. The PAMPA assay generates consistent, reproducible data over a range of permeability values.
To avoid non-specific binding, we’ve incorporated Corning GentestTM pre-coated PAMPA plates into our workflow. Unlike traditional PAMPA formulations, these plates are comprised of an oil-lipid-oil trilayer, offering a shortened path for compound diffusion more closely mimicking diffusion across the cell membrane while minimizing non-specific binding and improving the accuracy of PAMPA permeability values for compounds that are typically under-predicted using traditional PAMPA methods.
Ask us about obtaining solubility data alongside PAMPA for a more clear picture of your sample’s properties.
- Parallel Artificial Membrane Permeability using the Corning GentestTM pre-coated PAMPA plate system.
- 20 μL of 10 mM DMSO stock (HPLC-UV, HPLC-MS, and CLND)
- 200 μM (HPLC-UV and CLND)
- 10 μM (HPLC-MS)
- 5 hours (others available)
- PBS, pH 7.4
- Other media available
- Percent Retained
- Quotes available upon request