pKa

Using capillary electrophoresis, we can determine a compound’s ionization constants even for low-solubility molecules. Knowing your compound’s pKa is crucial for predicting solubility, permeability, and overall ADME performance in various pH environments.

pKa plays a significant role in a compound’s solubility, lipophilicity, and permeability

Capillary electrophoresis allows us to obtain pKa measurements for compounds with low solubility using a cosolvent approach. Unlike the potentiometric method, measurements can be obtained for compounds with chromophores located further away from the center of ionization.

Capillary electrophoresis separations are carried out in parallel across 24 equally spaced pH’s between 1.8 and 11.2. The compound migration time is monitored relative to a neutral marker (DMSO), and a titration curve is generated from this data.

An internal validation study was recently conducted to evaluate pKa measurements obtained at Analiza with those the from literature*.

 

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