In-Vitro ADME Properties
In-vitro ADME screening helps you prioritize and optimize compounds before costly in-vivo studies. At Analiza, our fast, flexible, and reliable assays provide critical insights into a drug candidate’s absorption, distribution, metabolism, and excretion, so you can eliminate weak leads and focus resources on your most promising compounds.
Why In-Vitro ADME Testing Matters
Predicting in-vivo performance early reduces late-stage failures and saves time and budget. By assessing microsomal stability, plasma stability, and membrane permeability, you can identify red flags upfront and refine your chemical series accordingly.
In-Vitro ADME Property Assays
Microsomal Stability
Determine how quickly your compound is metabolized by liver microsomes across various species (e.g., human, rat, mouse). Our HPLC-MS QTOF workflows capture full-scan data, eliminating the need for individual MS methods. Results include percent remaining or intrinsic clearance, giving you a clear picture of metabolic liabilities.
Plasma Stability
Uncover potential issues with rapid clearance or instability once a compound enters the bloodstream. We incubate your compounds in rat, mouse, dog, or human plasma and measure the rate of degradation over time. This assay is particularly relevant for prodrugs, where controlled plasma breakdown is desired.
PAMPA
Our Parallel Artificial Membrane Permeability Assay (PAMPA) evaluates passive diffusion across an artificial lipid membrane, mirroring the GI tract’s barrier. By capturing Papp and related parameters, you can quickly gauge a compound’s permeability profile and weed out poorly absorbed leads early.
Seamless Integration with Physicochemical Data
Solubility issues can skew in-vitro ADME results, which is why we optimize each assay for low-solubility compounds. By pairing in-vitro ADME testing with our physicochemical property data, you gain a more complete understanding of how a compound might behave in a real biological environment.
Customized Screening Panels
Every project is different. We can bundle multiple ADME assays—microsomal stability, plasma stability, PAMPA—into a tailored panel that aligns with your discovery goals. This approach streamlines logistics, shortens turnaround times, and provides a comprehensive data package for rapid decision-making.
Want to learn more about our services?
Contact our team today to see how Analiza can accelerate your drug discovery program.